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Anti-SALL4 Polyclonal Antibody

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Catalog No. TMAB-12480

Anti-SALL4 Polyclonal Antibody is a Rabbit antibody targeting SALL4. Anti-SALL4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SALL4 Polyclonal Antibody

Anti-SALL4 Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-12480
Anti-SALL4 Polyclonal Antibody is a Rabbit antibody targeting SALL4. Anti-SALL4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-SALL4 Polyclonal Antibody is a Rabbit antibody targeting SALL4. Anti-SALL4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SALL4 Polyclonal Antibody, Unconjugated (TMAB-12480) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-SALL4 (TMAB-12480) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 118 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityExpressed in testis.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundSall4 is mammalian homologs of the Drosophila region-specific homeotic gene spalt, which encodes a zinc finger-containing transcription regulator. Drosophila spalt is an essential genetic component required for the specification of posterior head and anterior tail as opposed to trunk. Sall3 is expressed at 24 weeks of gestation in several regions of the human fetal brain including neurons of the hippocampus formation and of mediodorsal and ventrolateral thalamic nuclei, Purkinje cells of the cerebellum, and a subset of neurons in the brainstem. Sall4 expression in early mouse embryos is gradually confined to the head region and the primitive streak, followed by prominent expression in the developing midbrain, branchial arches, limbs and genital papilla.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SALL4
Antigen Species
Human
Gene Name
SALL4
Gene ID
Protein Name
Sal-like protein 4
Uniprot ID
Function
Probable transcription factor.
Chemical Properties
Molecular WeightTheoretical: 112 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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