Anti-SAE1 Antibody (2I706) is a Rabbit antibody targeting SAE1. Anti-SAE1 Antibody (2I706) can be used in WB,ICC/IF,IP,FCM.

IgG

2I706

Human (predicted:Rat)

1. 25 μg total protein per lane of various lysates (see on figure) probed with SAE1 monoclonal antibody, unconjugated (TMAB-12472) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-SAE1 antibody (TMAB-12472, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human SAE1

Human

SAE1

SUMO-activating enzyme subunit 1

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.