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Anti-S1PR1 Antibody (9T480) (Synonyms: Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor...)
Catalog No. TMAC-01227 Copy Product Info
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Anti-S1PR1 Antibody (9T480) is a Rabbit antibody targeting S1PR1. Anti-S1PR1 Antibody (9T480) can be used in FCM,ICC/IF,IHC,WB.
Anti-S1PR1 Antibody (9T480)
Copy Product Info🥰Excellent
Catalog No. TMAC-01227
Synonyms Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor 1, S1PR 1, S1P1, S1P receptor Edg-1, S1P receptor 1, Endothelial differentiation G-protein coupled receptor 1, EDG1, CHEDG1, CD antigen CD363
Anti-S1PR1 Antibody (9T480) is a Rabbit antibody targeting S1PR1. Anti-S1PR1 Antibody (9T480) can be used in FCM,ICC/IF,IHC,WB.
Anti-S1PR1 Antibody
(9T480)
(9T480)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-S1PR1 Antibody (9T480) is a Rabbit antibody targeting S1PR1. Anti-S1PR1 Antibody (9T480) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | Sphingosine 1-phosphate receptor Edg-1, Sphingosine 1-phosphate receptor 1, S1PR 1, S1P1, S1P receptor Edg-1, S1P receptor 1, Endothelial differentiation G-protein coupled receptor 1, EDG1, CHEDG1, CD antigen CD363 |
| Ig Type | IgG |
| Clone | 9T480 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of EDG1 on SH-SY5Y cells lysates using anti-EDG1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse herat tissue using anti-EDG1 antibody. Counter stained with hematoxylin. 7. ICC staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. 11. All lanes: EDG1 Rabbit mAb at 1/1000 dilution, Lane 1: HUVEC whole cell lysates, Lane 2: JK whole cell lysates, Lane 3: HT29 whole cell lysates, Lane 4: Rat brain lysates Lysates at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution, Predicted band size: 43 kDa, Observed band size: 43-50 kDa, Exposure time: 5 seconds.            |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. EDG-6, which is expressed in lymphoid and hematopoietic tissues and in lung, signals through G(i/o) proteins, which activate growth related pathways |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 43 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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