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Anti-S100B Antibody (4A182)
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Catalog No. TMAC-03670
Alias S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in WB,ICC/IF,IP,IHC.
Anti-S100B Antibody (4A182)
Anti-S100B Antibody (4A182)
😃Good
Catalog No. TMAC-03670Alias S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in WB,ICC/IF,IP,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in WB,ICC/IF,IP,IHC. |
| Synonyms | S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF |
| Ig Type | IgG |
| Clone | 4A182 |
| Reactivity | Human,Mouse,Rat,Goat,zebrafish |
| Verified Activity | 1. Western blot analysis of S100 beta on different lysates using anti-S100 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver, Lane 2: Mouse heart, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin. 3. ICC staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbuminβ(also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 11 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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