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Anti-S100B Antibody (4A182) (Synonyms: S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF)

Catalog No. TMAC-03670 Copy Product Info
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Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in ICC/IF,IHC,IP,WB.

Anti-S100B Antibody (4A182)

Copy Product Info
🥰Excellent
Catalog No. TMAC-03670
Synonyms S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF

Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in ICC/IF,IHC,IP,WB.

Anti-S100B Antibody
(4A182)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in ICC/IF,IHC,IP,WB.
SynonymsS100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Ig Type
IgG
Clone
4A182
Reactivity
Human,Mouse,Rat,Goat,zebrafish
Verified Activity
1. Western blot analysis of S100 beta on different lysates using anti-S100 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver, Lane 2: Mouse heart, Lane 3: Hela.
2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin.
3. ICC staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbuminβ(also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 11 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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