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Anti-S100A9 Antibody (7L821)
😃Good
Catalog No. TMAY-01695
Alias S100 calcium binding protein A9, p14, MRP14, L1Ag, GAGB, Cagb, BEE22, AW546964, 60B8Ag
Anti-S100A9 Antibody (7L821) is a Mouse antibody targeting S100A9. Anti-S100A9 Antibody (7L821) can be used in FCM,ICC/IF.
Anti-S100A9 Antibody (7L821)
Anti-S100A9 Antibody (7L821)
😃Good
Catalog No. TMAY-01695Alias S100 calcium binding protein A9, p14, MRP14, L1Ag, GAGB, Cagb, BEE22, AW546964, 60B8Ag
Anti-S100A9 Antibody (7L821) is a Mouse antibody targeting S100A9. Anti-S100A9 Antibody (7L821) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-S100A9 Antibody (7L821) is a Mouse antibody targeting S100A9. Anti-S100A9 Antibody (7L821) can be used in FCM,ICC/IF. |
| Alias | S100 calcium binding protein A9, p14, MRP14, L1Ag, GAGB, Cagb, BEE22, AW546964, 60B8Ag |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 7L821 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human S100A9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A9 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus. 2. Flow cytometric analysis of Human S100A9 on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human S100A9 (Filled hisgram), then stained with a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by Anti-S100A9 Antibody was blocked by preincubation of the purified antibody with 20ug recombinant human S100A9 for 1 hour (Black solid line hisgram). |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A9 (rh S100A9; TMPY-01357; NP_002956.1; Met1-Pro114). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calciumbinding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. |
| Conjucates | Unconjugated |
| Others Formats | FITC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human S100A9 protein (TMPY-01357) |
| Antigen Species | Human |
| Biology Area | Calcium-binding Proteins and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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