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Anti-S100A6 Antibody (9N771)
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Catalog No. TMAC-03669
Alias S100 calcium binding protein A6, PRA, CACY, CABP, 5B10, 2A9
Anti-S100A6 Antibody (9N771) is a Rabbit antibody targeting S100A6. Anti-S100A6 Antibody (9N771) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-S100A6 Antibody (9N771)
Anti-S100A6 Antibody (9N771)
😃Good
Catalog No. TMAC-03669Alias S100 calcium binding protein A6, PRA, CACY, CABP, 5B10, 2A9
Anti-S100A6 Antibody (9N771) is a Rabbit antibody targeting S100A6. Anti-S100A6 Antibody (9N771) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-S100A6 Antibody (9N771) is a Rabbit antibody targeting S100A6. Anti-S100A6 Antibody (9N771) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | S100 calcium binding protein A6, PRA, CACY, CABP, 5B10, 2A9 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1,000 dilution. Positive control: Lane 1: A431, Lane 2: Mouse lung. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin. 7. ICC staining S100 alpha 6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining S100 alpha 6 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of SH-SY-5Y cells with S100 alpha 6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Calcyclin, also known as Prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9, S-100 calcium-binding protein A6 (S-100A6) or MLN 4, is a homodimeric member of the S-100 calcium-binding protein family whose expression is upregulated in proliferating and differentiating cells. Calcyclin is inducible by growth factors and overexpressed in acute myeloid leukemias. It is expressed in a cell-specific manner in subpopulations of neurons and astrocytes and in epithelial cells and fibroblasts. Calcyclin is a specific target of S-100B protein in vivo. The binding of Calcyclin to S-100B is stabilized by S-100B-bound calcium and zinc. Calcyclin associates with both Annexin XI and CacyBP (calcyclin-binding protein). It functions to activate several processes along the calcium signal transduction pathway including the regulation of cell growth, proliferation, secretion and exocytosis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 10 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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