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Anti-S100A6 Antibody (5Z514)
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Catalog No. TMAY-01355
Alias S100 calcium binding protein A6, PRA, CACY, CABP, 5B10, 2A9
Anti-S100A6 Antibody (5Z514) is a Mouse antibody targeting S100A6. Anti-S100A6 Antibody (5Z514) can be used in FCM,ICC/IF.
Anti-S100A6 Antibody (5Z514)
Anti-S100A6 Antibody (5Z514)
😃Good
Catalog No. TMAY-01355Alias S100 calcium binding protein A6, PRA, CACY, CABP, 5B10, 2A9
Anti-S100A6 Antibody (5Z514) is a Mouse antibody targeting S100A6. Anti-S100A6 Antibody (5Z514) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Chemical Properties
| Description | Anti-S100A6 Antibody (5Z514) is a Mouse antibody targeting S100A6. Anti-S100A6 Antibody (5Z514) can be used in FCM,ICC/IF. |
| Alias | S100 calcium binding protein A6, PRA, CACY, CABP, 5B10, 2A9 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 5Z514 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human S100A6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human S100A6 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human S100A6 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human S100A6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A6 (rh S100A6; TMPY-01339; NP_055439.1; Met1-Gly90). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. S100A6 (S100 calcium-binding protein A6) is a member of the S100 family of proteins, and functions in prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of S100A6 have been implicated in melanoma. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human S100A6 protein (TMPY-01339) |
| Antigen Species | Human |
| Biology Area | Calcium-binding Proteins and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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