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Anti-S100A4 Antibody (1T170)
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Catalog No. TMAB-12450
Anti-S100A4 Antibody (1T170) is a Mouse antibody targeting S100A4. Anti-S100A4 Antibody (1T170) can be used in IHC-P,IHC-Fr,IF.
Anti-S100A4 Antibody (1T170)
Anti-S100A4 Antibody (1T170)
Copy Product Info😃Good
Catalog No. TMAB-12450
Anti-S100A4 Antibody (1T170) is a Mouse antibody targeting S100A4. Anti-S100A4 Antibody (1T170) can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-S100A4 Antibody (1T170) is a Mouse antibody targeting S100A4. Anti-S100A4 Antibody (1T170) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG2a kappa |
| Clone | 1T170 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (S100A4) Monoclonal Antibody, Unconjugated (TMAB-12450) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Tissue Specificity | Ubiquitously expressed. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human S100A4 |
| Antigen Species | Human |
| Gene Name | S100A4 |
| Gene ID | |
| Protein Name | Protein S100-A4 |
| Uniprot ID |
| Molecular Weight | Theoretical: 12 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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