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Anti-S100A10 Antibody (3C599) is a Mouse antibody targeting S100A10. Anti-S100A10 Antibody (3C599) can be used in IHC-P,FCM,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $199 | 7-10 days |
Description | Anti-S100A10 Antibody (3C599) is a Mouse antibody targeting S100A10. Anti-S100A10 Antibody (3C599) can be used in IHC-P,FCM,ICC/IF. |
Alias | S100 calcium binding protein A10, P11, p10, MGC111133, GP11, CLP11, CAL1L, Ca[1], ANX2LG, ANX2L, 42C |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 3C599 |
Reactivity | Human |
Verified Activity | 1. Immunochemical staining of human S100A10 in human renal carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human S100A10 in human lung with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of human S100A10 in human breast carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). 4. Flow cytometric analysis of Human S100A10 expression on HT29 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human S100A10, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 5. Immunofluorescence staining of S100A10 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human S100A10 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
Application | IHC-P,FCM,ICC/IF |
Recommended Dose | IHC-P: 1:50-1:200; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A10 (rh S100A10; TMPY-01926; NP_002957.1; Pro2-Lys97). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S1 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A10, also known as Calpactin I light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11, ANX2LG and S100A10, is a member of the S100 family of small, dimeric EF hand-type Ca(2+)-binding proteins that generally modulate cellular target proteins in response to intracellular Ca(2+) signals. In contrast to all other S100 proteins, S100A10 is Ca(2+) insensitive because of amino acid replacements in its Ca(2+)-binding loops that lock the protein in a permanently active state. S100A10 forms a heterotetramer with annexin IIH and promotes carcinoma invasion and metastasis by plasminogen activation. S100A10 and annexin II contribute to the aggressive characteristics of anaplastic carcinoma, while playing a constitutive role in papillary carcinoma. S100A10 induces the dimerization of ANXA2 / p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target of tyrosine-specific kinase. S100A10 functions as a linker tethering certain transmembrane proteins to annexin A2 thereby assisting their traffic to the plasma membrane and/or their firm anchorage at certain membrane sites. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human S100A10 protein (TMPY-01926) |
Antigen Species | Human |
Biology Area | Calcium-binding Proteins and Related Molecules |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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