Anti-Runx3 Polyclonal Antibody 2 is a Rabbit antibody targeting Runx3. Anti-Runx3 Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Cow,Horse,Sheep)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated (TMAB-12430) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. A431 cells stained with Rabbit Anti-Runx3 Polyclonal Antibody at 4C overnight, followed by a Donkey Anti Rabbit FITC conjugated secondary antibody for 60 min in dark at RT

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Runx3

Human

RUNX3

Runt-related transcription factor 3

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.