Anti-Runx3 Polyclonal Antibody is a Rabbit antibody targeting Runx3. Anti-Runx3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Cow)

1. Sample: Lymph node (Mouse) Lysate at 30 μg
Primary: Anti-Runx3 (TMAB-12431) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
2. Sample: A549 Cell Lysate at 30 μg
Primary: Anti-Runx3 (TMAB-12431) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
3. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated (TMAB-12431) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Runx3

Human

RUNX3

Runt-related transcription factor 3

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.