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Anti-RRM2 Antibody (4A731)
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Catalog No. TMAB-12393
Anti-RRM2 Antibody (4A731) is a Mouse antibody targeting RRM2. Anti-RRM2 Antibody (4A731) can be used in WB,ICC/IF.
Anti-RRM2 Antibody (4A731)
Anti-RRM2 Antibody (4A731)
Copy Product Info😃Good
Catalog No. TMAB-12393
Anti-RRM2 Antibody (4A731) is a Mouse antibody targeting RRM2. Anti-RRM2 Antibody (4A731) can be used in WB,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-RRM2 Antibody (4A731) is a Mouse antibody targeting RRM2. Anti-RRM2 Antibody (4A731) can be used in WB,ICC/IF. |
| Ig Type | IgG1, Kappa |
| Clone | 4A731 |
| Reactivity | Human |
| Verified Activity | 1. Cell line: HepG2 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-12393 2. 25 ug total protein per lane of various lysates (see on figure) probed with RRM2 monoclonal antibody, unconjugated (TMAB-12393) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Ribonucleotide reductase is essential for the production and maintenance of the level of deoxyribonucleoside triphosphates (dNTPs) required for DNA synthesis. It is an enzymatic complex consisting of two nonidentical subunits, R1 and R2, which are inactive separately. R2, the smaller subunit, is localized to the cytoplasm. R2 is the limiting factor of the catalytic activity of the ribonucleotide reductase enzymatic complex. R2 expression is strictly correlated to the S-phase of the cell cycle, whereas R1 remains constant throughout all phases of the cell cycle. While R2 seems to be involved solely in the maintenance of dNTPs for DNA replication, a similar protein, p53R2, has been shown to be responsible for the production of dNTPs in response to DNA damage. Function : Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. |
| Gene Name | RRM2 |
| Gene ID | |
| Protein Name | Ribonucleoside-diphosphate reductase subunit M2 |
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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