Anti-RPS6 (N-term) Antibody (1F700) is a Mouse antibody targeting RPS6 (N-term). Anti-RPS6 (N-term) Antibody (1F700) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1F700

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with RPS6 (N-term) monoclonal antibody, unconjugated (TMAB-12367) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (TMAB-12367) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

Recombinant Protein: human RPS6

Human

RPS6

40S ribosomal protein S6

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.