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Anti-RPA1 Antibody (1L894)
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Catalog No. TMAC-03629
Alias Single-stranded DNA-binding protein, RPA70, RPA1, RP-A p70, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1 (RF-A protein 1), REPA1
Anti-RPA1 Antibody (1L894) is a Rabbit antibody targeting RPA1. Anti-RPA1 Antibody (1L894) can be used in WB,ICC,IF,FCM.
Anti-RPA1 Antibody (1L894)
Anti-RPA1 Antibody (1L894)
😃Good
Catalog No. TMAC-03629Alias Single-stranded DNA-binding protein, RPA70, RPA1, RP-A p70, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1 (RF-A protein 1), REPA1
Anti-RPA1 Antibody (1L894) is a Rabbit antibody targeting RPA1. Anti-RPA1 Antibody (1L894) can be used in WB,ICC,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-RPA1 Antibody (1L894) is a Rabbit antibody targeting RPA1. Anti-RPA1 Antibody (1L894) can be used in WB,ICC,IF,FCM. |
| Synonyms | Single-stranded DNA-binding protein, RPA70, RPA1, RP-A p70, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1 (RF-A protein 1), REPA1 |
| Ig Type | IgG |
| Clone | 1L894 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of RPA70 on Siha cell lysates using anti-RPA70 antibody at 1/500 dilution. 2. ICC staining RPA70 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining RPA70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining RPA70 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of 293T cells with RPA70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500; ICC: 1:50-100; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner. |
| Conjucates | Unconjugated |
| Uniprot ID |
| Molecular Weight | Theoretical: 68 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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