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Anti-ROCK2 Polyclonal Antibody
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Catalog No. TMAB-01628
Alias ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1
Anti-ROCK2 Polyclonal Antibody is a Rabbit antibody targeting ROCK2. Anti-ROCK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ROCK2 Polyclonal Antibody
Anti-ROCK2 Polyclonal Antibody
😃Good
Catalog No. TMAB-01628Alias ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1
Anti-ROCK2 Polyclonal Antibody is a Rabbit antibody targeting ROCK2. Anti-ROCK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ROCK2 Polyclonal Antibody is a Rabbit antibody targeting ROCK2. Anti-ROCK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Rabbit) |
| Verified Activity | 1. Sample: Muscle (Mouse) Lysate at 40 μg Primary: Anti-ROCK2 (TMAB-01628) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 161 kDa Observed band size: 161 kDa 2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ROCK2 Polyclonal Antibody, Unconjugated (TMAB-01628) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Mouse Heart Lysates Lane 2: Mouse Skeletal muscle Lysates Primary: Anti-ROCK2 (TMAB-01628) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 161 kDa Observed band size: 180 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human ROCK2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Serine/threonine kinases,Kinases,Other Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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