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Anti-ROCK2 Polyclonal Antibody

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Catalog No. TMAB-01628
Alias ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1

Anti-ROCK2 Polyclonal Antibody is a Rabbit antibody targeting ROCK2. Anti-ROCK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ROCK2 Polyclonal Antibody

Anti-ROCK2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01628Alias ROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1
Anti-ROCK2 Polyclonal Antibody is a Rabbit antibody targeting ROCK2. Anti-ROCK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-ROCK2 Polyclonal Antibody is a Rabbit antibody targeting ROCK2. Anti-ROCK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasROCK-II, ROCK 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, KIAA0619, EC 2.7.11.1
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Rabbit)
Verified Activity
1. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-ROCK2 (TMAB-01628) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kDa
Observed band size: 161 kDa
2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ROCK2 Polyclonal Antibody, Unconjugated (TMAB-01628) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Mouse Heart Lysates
Lane 2: Mouse Skeletal muscle Lysates
Primary: Anti-ROCK2 (TMAB-01628) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kDa
Observed band size: 180 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ROCK2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Serine/threonine kinases,Kinases,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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