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Anti-ROCK1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01627
Alias ROCK 1, Rho associated protein kinase 1, Rho associated coiled coil containing protein kinase 1, Renal carcinoma antigen NY REN 35, p160ROCK, p160 ROCK1

Anti-ROCK1 Polyclonal Antibody is a Rabbit antibody targeting ROCK1. Anti-ROCK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ROCK1 Polyclonal Antibody

Anti-ROCK1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01627Alias ROCK 1, Rho associated protein kinase 1, Rho associated coiled coil containing protein kinase 1, Renal carcinoma antigen NY REN 35, p160ROCK, p160 ROCK1
Anti-ROCK1 Polyclonal Antibody is a Rabbit antibody targeting ROCK1. Anti-ROCK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-ROCK1 Polyclonal Antibody is a Rabbit antibody targeting ROCK1. Anti-ROCK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasROCK 1, Rho associated protein kinase 1, Rho associated coiled coil containing protein kinase 1, Renal carcinoma antigen NY REN 35, p160ROCK, p160 ROCK1
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Rabbit)
Verified Activity
1. Sample:
Brain (Mouse) Lysate at 40 μg
Primary: Anti-ROCK1 (TMAB-01627) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 158 kDa
Observed band size: 160 kDa
2. Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ROCK1 Polyclonal Antibody, Unconjugated (TMAB-01627) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ROCK1 Polyclonal Antibody, Unconjugated (TMAB-01627) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-ROCK1 (TMAB-01627) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 160 kDa
Observed band size: 160 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ROCK1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cell division,Centromere,Actin Assembly,Other Kinases
Chemical Properties
Molecular WeightTheoretical: 158 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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