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Anti-RNF70 Polyclonal Antibody
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Catalog No. TMAB-12329
Anti-RNF70 Polyclonal Antibody is a Rabbit antibody targeting RNF70. Anti-RNF70 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-RNF70 Polyclonal Antibody
Anti-RNF70 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-12329
Anti-RNF70 Polyclonal Antibody is a Rabbit antibody targeting RNF70. Anti-RNF70 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-RNF70 Polyclonal Antibody is a Rabbit antibody targeting RNF70. Anti-RNF70 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Horse,Rabbit) |
| Verified Activity | 1. Sample: 293T (Human) Cell Lysate at 30 μg HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-RNF70 (TMAB-12329) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 71 kD Observed band size: 71 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RNF70) Polyclonal Antibody, Unconjugated (TMAB-12329) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. |
| Immunogen | KLH conjugated synthetic peptide: human RNF70 |
| Antigen Species | Human |
| Gene Name | PJA1 |
| Gene ID | |
| Protein Name | E3 ubiquitin-protein ligase Praja-1 |
| Uniprot ID | |
| Function | Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting. |
| Molecular Weight | Theoretical: 71 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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