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Anti-RNF36 Polyclonal Antibody

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Catalog No. TMAB-12319

Anti-RNF36 Polyclonal Antibody is a Rabbit antibody targeting RNF36. Anti-RNF36 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-RNF36 Polyclonal Antibody

Anti-RNF36 Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-12319
Anti-RNF36 Polyclonal Antibody is a Rabbit antibody targeting RNF36. Anti-RNF36 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-RNF36 Polyclonal Antibody is a Rabbit antibody targeting RNF36. Anti-RNF36 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow,Sheep)
Verified Activity
1. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-RNF36 (TMAB-12319) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RNF36) Polyclonal Antibody, Unconjugated (TMAB-12319) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RNF36) Polyclonal Antibody, Unconjugated (TMAB-12319) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus speckle
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RNF36
Antigen Species
Human
Gene Name
TRIM69
Gene ID
Protein Name
E3 ubiquitin-protein ligase TRIM69
Function
May play a role in apoptosis.
Chemical Properties
Molecular WeightTheoretical: 57 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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