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Anti-RNF210 Polyclonal Antibody

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Catalog No. TMAB-12312

Anti-RNF210 Polyclonal Antibody is a Rabbit antibody targeting RNF210. Anti-RNF210 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-RNF210 Polyclonal Antibody

Anti-RNF210 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-12312
Anti-RNF210 Polyclonal Antibody is a Rabbit antibody targeting RNF210. Anti-RNF210 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-RNF210 Polyclonal Antibody is a Rabbit antibody targeting RNF210. Anti-RNF210 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Pig,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RNF210) Polyclonal Antibody, Unconjugated (TMAB-12312) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-RNF210 (TMAB-12312) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
3. Sample: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-RNF210 (TMAB-12312) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 33 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundRFPL4A is a 287 amino acid protein. RFPL4A contains a cysteine-rich RING finger-like region (C3YC4), a coiled-coil motif, and B30.2 domains characteristic of the RING-B30 family of E3 ubiquitin-protein ligases, such as MID1. The RFPL4A gene maps to human chromosome 19q13.42. Chromosome 19 consists of approximately 63 million bases and makes up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families, and Fc?receptors. Key genes for eye color and hair color also map to chromosome 19. Peutz-Jeghers syndrome, spinocerebellar ataxia type 6, the stroke disorder CADASIL, hypercholesterolemia and insulin-dependent diabetes have been linked to chromosome 19.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RFPL4A/RNF210
Antigen Species
Human
Gene Name
RFPL4A
Gene ID
Protein Name
Ret finger protein-like 4A
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 32 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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