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Anti-RNF20 Polyclonal Antibody

Catalog No. TMAB-12309 Copy Product Info
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Anti-RNF20 Polyclonal Antibody is a Rabbit antibody targeting RNF20. Anti-RNF20 Polyclonal Antibody can be used in WB.

Anti-RNF20 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-12309

Anti-RNF20 Polyclonal Antibody is a Rabbit antibody targeting RNF20. Anti-RNF20 Polyclonal Antibody can be used in WB.

Anti-RNF20 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-RNF20 Polyclonal Antibody is a Rabbit antibody targeting RNF20. Anti-RNF20 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
Sample:
HepG2 Cell (Human) Lysate at 30 μg
Raji Cell (Human) Lysate at 30 μg
Primary: Anti-RNF20 (TMAB-12309) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 4 kD
Observed band size: 11 4 kD
Application
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNuclear
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundRNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes. It is recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RNF20
Antigen Species
Human
Gene Name
RNF20
Gene ID
Protein Name
E3 ubiquitin-protein ligase BRE1A
Uniprot ID
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Chemical Properties
Molecular WeightTheoretical: 114 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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