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Anti-RNF20 Polyclonal Antibody

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Catalog No. TMAB-12309

Anti-RNF20 Polyclonal Antibody is a Rabbit antibody targeting RNF20. Anti-RNF20 Polyclonal Antibody can be used in WB.

Anti-RNF20 Polyclonal Antibody

Anti-RNF20 Polyclonal Antibody

😃Good
Catalog No. TMAB-12309
Anti-RNF20 Polyclonal Antibody is a Rabbit antibody targeting RNF20. Anti-RNF20 Polyclonal Antibody can be used in WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-RNF20 Polyclonal Antibody is a Rabbit antibody targeting RNF20. Anti-RNF20 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNuclear
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundRNF20 is an E3 ubiquitin ligase protein that mediates monoubiquitination of histone H2B and the methylation of histone H3. It forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. It is required for transcriptional activation of Hox genes. It is recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RNF20
Antigen Species
Human
Gene Name
RNF20
Gene ID
56254
Protein Name
E3 ubiquitin-protein ligase BRE1A
Uniprot ID
Q5VTR2
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Chemical Properties
Molecular WeightTheoretical: 114 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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