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Anti-RNF20 Antibody (4H387)

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Catalog No. TMAB-12310

Anti-RNF20 Antibody (4H387) is a Rabbit antibody targeting RNF20. Anti-RNF20 Antibody (4H387) can be used in WB,ICC/IF,IP,FCM.

Anti-RNF20 Antibody (4H387)

Anti-RNF20 Antibody (4H387)

😃Good
Catalog No. TMAB-12310
Anti-RNF20 Antibody (4H387) is a Rabbit antibody targeting RNF20. Anti-RNF20 Antibody (4H387) can be used in WB,ICC/IF,IP,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-RNF20 Antibody (4H387) is a Rabbit antibody targeting RNF20. Anti-RNF20 Antibody (4H387) can be used in WB,ICC/IF,IP,FCM.
Ig Type
IgG
Clone
4H387
Reactivity
Human (predicted:Mouse)
Application
WB
ICC/IF
IP
FCM
Recommended Dose
WB: 1:1000-2000; ICC/IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNuclear
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation.
Antigen Details
Immunogen
A synthesized peptide: human RNF20
Antigen Species
Human
Gene Name
RNF20
Gene ID
56254
Protein Name
E3 ubiquitin-protein ligase BRE1A
Uniprot ID
Q5VTR2
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator.
Chemical Properties
Molecular WeightTheoretical: 114 kDa. Actual: 114 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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