Anti-RNF156 Polyclonal Antibody is a Rabbit antibody targeting RNF156. Anti-RNF156 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (TMAB-12300) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (TMAB-12300) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human RNF156

Human

MGRN1

E3 ubiquitin-protein ligase MGRN1

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination.