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Anti-RNASE4 Polyclonal Antibody 2 is a Rabbit antibody targeting RNASE4. Anti-RNASE4 Polyclonal Antibody 2 can be used in WB,IHC-P.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-RNASE4 Polyclonal Antibody 2 is a Rabbit antibody targeting RNASE4. Anti-RNASE4 Polyclonal Antibody 2 can be used in WB,IHC-P. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-RNASE4/Angiogenin Polyclonal Antibody, Unconjugated (TMAB-12274) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HUVEC cell lysates Primary: Anti-RNASE4 (TMAB-12274) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 15 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene belongs to the pancreatic ribonuclease family. It plays an important role in mRNA cleavage and has marked specificity towards the 3' side of uridine nucleotides. Alternative splicing results in four transcript variants encoding the same protein. This gene and the gene that encodes angiogenin share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2013]. |
| Immunogen | KLH conjugated synthetic peptide: human RNASE4 |
| Antigen Species | Human |
| Gene Name | RNASE4 |
| Gene ID | |
| Protein Name | Ribonuclease 4 |
| Uniprot ID | |
| Function | This RNase has marked specificity towards the 3' side of uridine nucleotides. |
| Molecular Weight | Theoretical: 14 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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