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Anti-RIPK3 Polyclonal Antibody

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Catalog No. TMAB-01622

Anti-RIPK3 Polyclonal Antibody is a Rabbit antibody targeting RIPK3. Anti-RIPK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-RIPK3 Polyclonal Antibody

Anti-RIPK3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01622
Anti-RIPK3 Polyclonal Antibody is a Rabbit antibody targeting RIPK3. Anti-RIPK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-RIPK3 Polyclonal Antibody is a Rabbit antibody targeting RIPK3. Anti-RIPK3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,GuineaPig)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RIPK3) Polyclonal Antibody, Unconjugated (TMAB-01622) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
2. Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-RIPK3 Polyclonal Antibody, Unconjugated (TMAB-01622) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample: A549 cells lysates, 30 μg;
Primary: Anti-RIPK3 (TMAB-01622) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 57 kDa
Observed band size: 57 kDa
4. Sample:
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-RIPK3 (TMAB-01622) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
5. Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human K562 cell lysates
Lane 3: Human Raji cell lysates
Primary: Anti-RIPK3 (TMAB-01622) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cell membrane. Mitochondrion (Potential).
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human RIPK3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Associated Proteins,Necroptosis,Kinases,Associated Proteins,TLR Signaling,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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