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Anti-RIG-I Polyclonal Antibody
😃Good
Catalog No. TMAB-01619
Anti-RIG-I Polyclonal Antibody is a Rabbit antibody targeting RIG-I. Anti-RIG-I Polyclonal Antibody can be used in WB.
Anti-RIG-I Polyclonal Antibody
Anti-RIG-I Polyclonal Antibody
😃Good
Catalog No. TMAB-01619
Anti-RIG-I Polyclonal Antibody is a Rabbit antibody targeting RIG-I. Anti-RIG-I Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-RIG-I Polyclonal Antibody is a Rabbit antibody targeting RIG-I. Anti-RIG-I Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Dog,Pig,Cow) |
| Verified Activity | Sample: Raw264.7 (Mouse) Cell Lysate at 30 μg Primary: Anti-DDX58 (TMAB-01619) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 106 kDa Observed band size: 114 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1/DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1/DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1/DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus. |
| Immunogen | KLH conjugated synthetic peptide: human DDX58 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | DNA / RNA binding,RNA Processing,Antiviral Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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