Anti-RhoGDI 1 Antibody (6G946) is a Rabbit antibody targeting RhoGDI 1. Anti-RhoGDI 1 Antibody (6G946) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

6G946

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with RhoGDI 1 monoclonal antibody, unconjugated (TMAB-12248) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RhoGDI 1 antibody (TMAB-12248, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human RhoGDI 1

Human

ARHGDIA

Rho GDP-dissociation inhibitor 1

Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.