Anti-RhoGAP5 Antibody (1Z828) is a Rabbit antibody targeting RhoGAP5. Anti-RhoGAP5 Antibody (1Z828) can be used in WB,IHC-P,IHC-Fr,FCM,IF.

IgG

1Z828

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RhoGAP5 Monoclonal Antibody, Unconjugated (TMAB-12247) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RhoGAP5 Monoclonal Antibody, Unconjugated (TMAB-12247) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RhoGAP5 Monoclonal Antibody, Unconjugated (TMAB-12247) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with RhoGAP5 monoclonal antibody, unconjugated (TMAB-12247) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; FCM: 1:50-100; IF: 1:50-200

Monoclonal

A synthesized peptide: human ARHGAP5

Human

ARHGAP5

Rho GTPase-activating protein 5

GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of RASA1/p120GAP.