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Anti-Rhodopsin Antibody (5W703)

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Catalog No. TMAB-12244 Copy Product Info
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Anti-Rhodopsin Antibody (5W703) is a Rabbit antibody targeting Rhodopsin. Anti-Rhodopsin Antibody (5W703) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Rhodopsin Antibody
(5W703)

Pack SizePriceUSA StockGlobal StockQuantity
25 µL$1497-10 days7-10 days
50 µL$2627-10 days7-10 days
100 µL$4707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Rhodopsin Antibody (5W703) is a Rabbit antibody targeting Rhodopsin. Anti-Rhodopsin Antibody (5W703) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
5W703
Reactivity
Rat (predicted:Human)
Verified Activity
1. Western blot analysis of rat eyeball lysate. Using Rhodopsin (TMAB-12244) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Rhodopsin Monoclonal Antibody, Unconjugated (TMAB-12244) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
Tissue SpecificityRod shaped photoreceptor cells which mediates vision in dim light.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundPhotoreceptor required for image-forming vision at low light intensity.Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins.
Antigen Details
Immunogen
A synthesized peptide: human Rhodopsin
Antigen Species
Human
Gene Name
RHO
Gene ID
Protein Name
Rhodopsin
Uniprot ID
Function
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
Chemical Properties
Molecular WeightTheoretical: 39 kDa. Actual: 39 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-Rhodopsin Antibody (5W703) molecular weight