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Anti-RheB Antibody (8S39)

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Catalog No. TMAC-03596
Alias RHEB2, Ras homolog enriched in brain

Anti-RheB Antibody (8S39) is a Rabbit antibody targeting RheB. Anti-RheB Antibody (8S39) can be used in WB,ICC,IF,IHC,FCM.

Anti-RheB Antibody (8S39)

Anti-RheB Antibody (8S39)

😃Good
Catalog No. TMAC-03596Alias RHEB2, Ras homolog enriched in brain
Anti-RheB Antibody (8S39) is a Rabbit antibody targeting RheB. Anti-RheB Antibody (8S39) can be used in WB,ICC,IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-RheB Antibody (8S39) is a Rabbit antibody targeting RheB. Anti-RheB Antibody (8S39) can be used in WB,ICC,IF,IHC,FCM.
SynonymsRHEB2, Ras homolog enriched in brain
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of RHEB on mouse placenta tissue lysate using anti-RHEB antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RHEB antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RHEB antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RHEB antibody. Counter stained with hematoxylin.
5. ICC staining RHEB in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining RHEB in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of A431 cells with RHEB antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundH-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein), also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human RHEB aa 1-250
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 20 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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