Anti-RGS17 Polyclonal Antibody is a Rabbit antibody targeting RGS17. Anti-RGS17 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-RGS17 Polyclonal Antibody, Unconjugated (TMAB-12225) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RGS17) Polyclonal Antibody, Unconjugated (TMAB-12225) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human RGS17

Human

RGS17

Regulator of G-protein signaling 17

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.