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Anti-RFX4 Polyclonal Antibody
😃Good
Catalog No. TMAB-12218
Anti-RFX4 Polyclonal Antibody is a Rabbit antibody targeting RFX4. Anti-RFX4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-RFX4 Polyclonal Antibody
Anti-RFX4 Polyclonal Antibody
😃Good
Catalog No. TMAB-12218
Anti-RFX4 Polyclonal Antibody is a Rabbit antibody targeting RFX4. Anti-RFX4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-RFX4 Polyclonal Antibody is a Rabbit antibody targeting RFX4. Anti-RFX4 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,GuineaPig) |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nuclear |
| Tissue Specificity | Isoform 4 is testis-specific. Isoform 1 is expressed in brain and gliomas. Isoform 2 and isoform 3 are testis-specific (at protein level). Isoform 1 is expressed in brain (at protein level). Isoform 3 is expressed at a higher level in adult testes and ejaculated spematozoa than in fetal testes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | EP and EP-like sites are regulatory enhancer elements found in the promoters of several viral and mammalian genes which, in humans, include the MIF-1 binding site (MIE) of the c-Myc gene, the X box of MHC class II promoters and a binding site in the PCNA (proliferating cell nuclear antigen) promoter. The EP-like sites present in the X box of MHC class II promoters are distinctly nonpalindromic sequences that contain only a single EP-homologous half-site. The EP-like element is bound by a ubiquitous nuclear protein complex that consists of homo- and heterodimers involving the RFX1, RFX2, RFX3, RFX4 and RFX5 proteins. The RFX proteins represent an essential class II transcription factor family that share several conserved regions, including a centrally located DNA-binding domain (DBD) and a C-terminal D region that facilitates dimerization. RFX4 is a 735 amino acid nuclear protein that, via interactions with other RFX proteins, can bind DNA and is thought to activate the transcription of target genes. Four isoforms, each of which exhibit different tissue specificity, exist due to alternative splicing events. |
| Immunogen | KLH conjugated synthetic peptide: human RFX4 |
| Antigen Species | Human |
| Gene Name | RFX4 |
| Gene ID | |
| Protein Name | Transcription factor RFX4 |
| Uniprot ID | |
| Function | RFX4 is a transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. RFX4 may be a transcriptional repressor rather than a transcriptional activator.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene. |
| Molecular Weight | Theoretical: 83 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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