Anti-RCL Polyclonal Antibody is a Rabbit antibody targeting RCL. Anti-RCL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Horse,Rabbit)

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-RCL/c Myc responsive Polyclonal Antibody, Unconjugated (TMAB-12168) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Kidney (Rat) Lysate at 40 μg
Liver (Rat) Lysate at 40 μg
Primary: Anti-RCL (TMAB-12168) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
We are unsure as to the identity of these extra bands
May be formed of homo-dimer

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human RCL/c Myc responsive

Human

DNPH1

2'-deoxynucleoside 5'-phosphate N-hydrolase 1 2'-deoxynucleoside 5'-phosphate N-hydrolase 1

Catalyzes the cleavage of the N-glycosidic bond ofdeoxyribonucleoside 5'-monophosphates to yield deoxyribose5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside5'-monophosphates containing purine bases are preferred to thosecontaining pyrimidine bases (By similarity).