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Anti-RASSF3 Polyclonal Antibody
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Catalog No. TMAB-12120
Anti-RASSF3 Polyclonal Antibody is a Rabbit antibody targeting RASSF3. Anti-RASSF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-RASSF3 Polyclonal Antibody
Anti-RASSF3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-12120
Anti-RASSF3 Polyclonal Antibody is a Rabbit antibody targeting RASSF3. Anti-RASSF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-RASSF3 Polyclonal Antibody is a Rabbit antibody targeting RASSF3. Anti-RASSF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: A549 Cell (Human) Lysate at 40 μg Primary: Anti-RASSF3 (TMAB-12120) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 26 kD Observed band size: 26 kD 2. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RASSF3) Polyclonal Antibody, Unconjugated (TMAB-12120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Stomach (Mouse) Lysate at 40 μg Primary: Anti-RASSF3 (TMAB-12120) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 30 kD 4. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RASSF3) Polyclonal Antibody, Unconjugated (TMAB-12120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RASSF3) Polyclonal Antibody, Unconjugated (TMAB-12120) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Note=Localized to microtubules in vascular endothelial cells. |
| Tissue Specificity | Widely expressed. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]. |
| Immunogen | KLH conjugated synthetic peptide: human RASSF3 |
| Antigen Species | Human |
| Gene Name | RASSF3 |
| Gene ID | |
| Protein Name | Ras association domain-containing protein 3 |
| Uniprot ID |
| Molecular Weight | Theoretical: 26 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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