Anti-RASAL2 Polyclonal Antibody is a Rabbit antibody targeting RASAL2. Anti-RASAL2 Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse)

1. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 3: MKN45 (Human) Cell Lysate at 30 μg
Lane 4: HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-RASAL2 (TMAB-12106) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 170 kD
2. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-RASAL2 antibody (TMAB-12106)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human RASAL2

Human

RASAL2

Ras GTPase-activating protein nGAP

Inhibitory regulator of the Ras-cyclic AMP pathway.