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Anti-RASAL2 Polyclonal Antibody
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Catalog No. TMAB-12106 Copy Product Info
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Anti-RASAL2 Polyclonal Antibody is a Rabbit antibody targeting RASAL2. Anti-RASAL2 Polyclonal Antibody can be used in WB,FCM.
Anti-RASAL2 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $223 | 7-10 days | 7-10 days | |
| 100 µL | $372 | 7-10 days | 7-10 days | |
| 200 µL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-RASAL2 Polyclonal Antibody is a Rabbit antibody targeting RASAL2. Anti-RASAL2 Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse) |
| Verified Activity | 1. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: MDA-MB-231 (Human) Cell Lysate at 30 μg Lane 3: MKN45 (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-RASAL2 (TMAB-12106) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129 kD Observed band size: 170 kD 2. Blank control: A431. Primary Antibody (green line): Rabbit Anti-RASAL2 antibody (TMAB-12106) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a protein that contains the GAP-related domain (GRD), a characteristic domain of GTPase-activating proteins (GAPs). GAPs function as activators of Ras superfamily of small GTPases. The protein encoded by this gene is able to complement the defective RasGAP function in a yeast system. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human RASAL2 |
| Antigen Species | Human |
| Gene Name | RASAL2 |
| Gene ID | |
| Protein Name | Ras GTPase-activating protein nGAP |
| Uniprot ID | |
| Function | Inhibitory regulator of the Ras-cyclic AMP pathway. |
| Molecular Weight | Theoretical: 129 kDa. Actual: 170 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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