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Anti-RAR gamma Polyclonal Antibody
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Catalog No. TMAB-12096
Anti-RAR gamma Polyclonal Antibody is a Rabbit antibody targeting RAR gamma. Anti-RAR gamma Polyclonal Antibody can be used in WB,ICC/IF.
Anti-RAR gamma Polyclonal Antibody
Anti-RAR gamma Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-12096
Anti-RAR gamma Polyclonal Antibody is a Rabbit antibody targeting RAR gamma. Anti-RAR gamma Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-RAR gamma Polyclonal Antibody is a Rabbit antibody targeting RAR gamma. Anti-RAR gamma Polyclonal Antibody can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human A549 cell lysates Primary: Anti-RAR gamma (TMAB-12096) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 48 kDa 2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (RAR gamma) polyclonal Antibody, Unconjugated (TMAB-12096) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. |
| Immunogen | KLH conjugated synthetic peptide: human Retinoic Acid Receptor gamma |
| Antigen Species | Human |
| Gene Name | RARG |
| Gene ID | |
| Protein Name | Retinoic acid receptor gamma |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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