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Anti-RAP80 Antibody (7N70)
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Catalog No. TMAB-12089
Anti-RAP80 Antibody (7N70) is a Rabbit antibody targeting RAP80. Anti-RAP80 Antibody (7N70) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-RAP80 Antibody (7N70)
Anti-RAP80 Antibody (7N70)
Copy Product Info😃Good
Catalog No. TMAB-12089
Anti-RAP80 Antibody (7N70) is a Rabbit antibody targeting RAP80. Anti-RAP80 Antibody (7N70) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-RAP80 Antibody (7N70) is a Rabbit antibody targeting RAP80. Anti-RAP80 Antibody (7N70) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Clone | 7N70 |
| Reactivity | Human |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with RAP80 monoclonal antibody, unconjugated (TMAB-12089) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RAP80 antibody (TMAB-12089, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Ubiquitin-binding protein.Specifically recognizes and binds 'Lys-63'-linked ubiquitin.Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). |
| Immunogen | A synthesized peptide: human RAP80 |
| Antigen Species | Human |
| Gene Name | UIMC1 |
| Gene ID | |
| Protein Name | BRCA1-A complex subunit RAP80 |
| Uniprot ID |
| Molecular Weight | Theoretical: 80 kDa. Actual: 80 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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