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Anti-RAP1GAP Antibody (4C780)
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Catalog No. TMAC-03533
Alias RPGP1_HUMAN, RAPGAP, RAP1GAPII, Rap1GAP1, Rap1GAP, Rap1ga1 protein, RAP1GA1, Rap1 GTPase-activating protein 1, Rap1 GTPase activating protein 1, RAP1 GTPase activating protein, KIAA0474
Anti-RAP1GAP Antibody (4C780) is a Mouse antibody targeting RAP1GAP. Anti-RAP1GAP Antibody (4C780) can be used in WB,FCM.
Anti-RAP1GAP Antibody (4C780)
Anti-RAP1GAP Antibody (4C780)
😃Good
Catalog No. TMAC-03533Alias RPGP1_HUMAN, RAPGAP, RAP1GAPII, Rap1GAP1, Rap1GAP, Rap1ga1 protein, RAP1GA1, Rap1 GTPase-activating protein 1, Rap1 GTPase activating protein 1, RAP1 GTPase activating protein, KIAA0474
Anti-RAP1GAP Antibody (4C780) is a Mouse antibody targeting RAP1GAP. Anti-RAP1GAP Antibody (4C780) can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $427 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-RAP1GAP Antibody (4C780) is a Mouse antibody targeting RAP1GAP. Anti-RAP1GAP Antibody (4C780) can be used in WB,FCM. |
| Synonyms | RPGP1_HUMAN, RAPGAP, RAP1GAPII, Rap1GAP1, Rap1GAP, Rap1ga1 protein, RAP1GA1, Rap1 GTPase-activating protein 1, Rap1 GTPase activating protein 1, RAP1 GTPase activating protein, KIAA0474 |
| Clone | 4C780 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1,000 dilution. 2. Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1,000 dilution. 3. Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gα z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, and also attenuates the ability of activated Gα z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gα z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 73 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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