Anti-RANKL/CD254 Polyclonal Antibody 2 is a Rabbit antibody targeting RANKL/CD254. Anti-RANKL/CD254 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Horse,Rabbit)

1. Blank control: HL-60.
Primary Antibody (green line): Rabbit Anti-RANKL/CD254 antibody (TMAB-12082)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lymph node (Mouse) Lysate at 40 μg
Lymph node (Rat) Lysate at 40 μg
Primary: Anti-RANKL’CD254 (TMAB-12082) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37’50 kD
Observed band size: 50 kD
3. Sample:
U937 (Human) Cell Lysate at 40 μg
Huvec (Human) Cell Lysate at 40 μg
Primary: Anti-RANKL/CD254 (TMAB-12082) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human RANKL/CD254

Human

TNFSF11

Tumor necrosis factor ligand superfamily member 11

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.