Anti-RANGAP1 Antibody (5I240) is a Rabbit antibody targeting RANGAP1. Anti-RANGAP1 Antibody (5I240) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

5I240

Human (predicted:Mouse,Rat)

1. 25 ug total protein per lane of various lysates (see on figure) probed with RANGAP1 monoclonal antibody, unconjugated (TMAB-12079) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RANGAP1 antibody (TMAB-12079, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (RANGAP1) monoclonal Antibody, unconjugated (TMAB-12079) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human RANGAP1

Human

RANGAP1

Ran GTPase-activating protein 1

GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.