Anti-RANBP9 Antibody (9K369) is a Rabbit antibody targeting RANBP9. Anti-RANBP9 Antibody (9K369) can be used in FCM, ICC/IF, WB.

IgG

9K369

Human,Mouse,Rat

1. Western blot analysis of HeLa cell lysate. Using RANBP9 (TMAB-12078) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RANBP9 antibody (TMAB-12078, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; WB=1:1000-2000

Monoclonal

A synthesized peptide: human RanBP9

Human

RANBP9

RAN binding protein 9

May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. May be involved in signaling of ITGB2/LFA-1 and other integrins. Enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but not affect estrogen-induced transactivation. Stabilizes TP73 isoform Alpha, probably by inhibiting its ubiquitination, and increases its proapoptotic activity. Inhibits the kinase activity of DYRK1A and DYRK1B. Inhibits FMR1 binding to RNA.