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Anti-RAGE Antibody (3H335)
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Catalog No. TMAC-03528
Alias Receptor for advanced glycosylation end products, Advanced glycosylation end product-specific receptor
Anti-RAGE Antibody (3H335) is a Rabbit antibody targeting RAGE. Anti-RAGE Antibody (3H335) can be used in WB,ICC/IF,IHC,FCM.
Anti-RAGE Antibody (3H335)
Anti-RAGE Antibody (3H335)
😃Good
Catalog No. TMAC-03528Alias Receptor for advanced glycosylation end products, Advanced glycosylation end product-specific receptor
Anti-RAGE Antibody (3H335) is a Rabbit antibody targeting RAGE. Anti-RAGE Antibody (3H335) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-RAGE Antibody (3H335) is a Rabbit antibody targeting RAGE. Anti-RAGE Antibody (3H335) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Receptor for advanced glycosylation end products, Advanced glycosylation end product-specific receptor |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin. 3. ICC staining RAGE in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining RAGE in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Jurkat cells with RAGE antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products). RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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