Anti-RAD52 Antibody (3H313) is a Rabbit antibody targeting RAD52. Anti-RAD52 Antibody (3H313) can be used in WB,FCM.

IgG

3H313

Human

1. Western blot analysis of PC3 cell lysate. Using RAD52 (TMAB-12047) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-RAD52 antibody (TMAB-12047, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; FCM: 1:50-100

Monoclonal

A synthesized peptide: human RAD52

Human

RAD52

DNA repair protein RAD52 homolog

Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.