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Anti-RAD50 Antibody (2B794) is a Rabbit antibody targeting RAD50. Anti-RAD50 Antibody (2B794) can be used in WB,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
| Description | Anti-RAD50 Antibody (2B794) is a Rabbit antibody targeting RAD50. Anti-RAD50 Antibody (2B794) can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Clone | 2B794 |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents. |
| Tissue Specificity | Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis.The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. |
| Immunogen | A synthesized peptide: human RAD50 |
| Antigen Species | Human |
| Gene Name | RAD50 |
| Gene ID | |
| Protein Name | DNA repair protein RAD50 |
| Uniprot ID | |
| Function | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. |
| Molecular Weight | Theoretical: 154 kDa. Actual: 154 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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