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Anti-RAD18 Antibody (5U394)
😃Good
Catalog No. TMAC-03504
Alias YCR66W, YCR066W, Structural maintenance of chromosomes protein 6, RNF73, RNF 73, RING finger protein 73, Radiation sensitivity protein 18, Rad18sc, RAD18_HUMAN, RAD18 transcript variant, RAD18 S. cerevisiae homolog of, RAD18 S. cerevisiae homolog, RAD18 homolog (S. cerevisiae), RAD18 homolog, RAD 18, Postreplication repair protein RAD18, Postreplication repair protein hRAD18p, Postreplication repair E3 ubiquitin-protein ligase RAD18, MGC156682, hRAD18, hRAD 18, hHR18, hHR 18, EC 6.3.2.-, E3 ubiquitin-protein ligase RAD18, DNA repair protein rad18, 2810024C04Rik
Anti-RAD18 Antibody (5U394) is a Rabbit antibody targeting RAD18. Anti-RAD18 Antibody (5U394) can be used in WB,ICC/IF,IHC,FCM.
Anti-RAD18 Antibody (5U394)
Anti-RAD18 Antibody (5U394)
😃Good
Catalog No. TMAC-03504Alias YCR66W, YCR066W, Structural maintenance of chromosomes protein 6, RNF73, RNF 73, RING finger protein 73, Radiation sensitivity protein 18, Rad18sc, RAD18_HUMAN, RAD18 transcript variant, RAD18 S. cerevisiae homolog of, RAD18 S. cerevisiae homolog, RAD18 homolog (S. cerevisiae), RAD18 homolog, RAD 18, Postreplication repair protein RAD18, Postreplication repair protein hRAD18p, Postreplication repair E3 ubiquitin-protein ligase RAD18, MGC156682, hRAD18, hRAD 18, hHR18, hHR 18, EC 6.3.2.-, E3 ubiquitin-protein ligase RAD18, DNA repair protein rad18, 2810024C04Rik
Anti-RAD18 Antibody (5U394) is a Rabbit antibody targeting RAD18. Anti-RAD18 Antibody (5U394) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-RAD18 Antibody (5U394) is a Rabbit antibody targeting RAD18. Anti-RAD18 Antibody (5U394) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | YCR66W, YCR066W, Structural maintenance of chromosomes protein 6, RNF73, RNF 73, RING finger protein 73, Radiation sensitivity protein 18, Rad18sc, RAD18_HUMAN, RAD18 transcript variant, RAD18 S. cerevisiae homolog of, RAD18 S. cerevisiae homolog, RAD18 homolog (S. cerevisiae), RAD18 homolog, RAD 18, Postreplication repair protein RAD18, Postreplication repair protein hRAD18p, Postreplication repair E3 ubiquitin-protein ligase RAD18, MGC156682, hRAD18, hRAD 18, hHR18, hHR 18, EC 6.3.2.-, E3 ubiquitin-protein ligase RAD18, DNA repair protein rad18, 2810024C04Rik |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3, Lane 2: 293T, Lane 3: MCF-7. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin. 3. ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 56/90 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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