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Anti-RAB5C Antibody (8Z372)

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Catalog No. TMAB-12006

Anti-RAB5C Antibody (8Z372) is a Rabbit antibody targeting RAB5C. Anti-RAB5C Antibody (8Z372) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-RAB5C Antibody (8Z372)

Anti-RAB5C Antibody (8Z372)

Copy Product Info
😃Good
Catalog No. TMAB-12006
Anti-RAB5C Antibody (8Z372) is a Rabbit antibody targeting RAB5C. Anti-RAB5C Antibody (8Z372) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2617-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-RAB5C Antibody (8Z372) is a Rabbit antibody targeting RAB5C. Anti-RAB5C Antibody (8Z372) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Clone
8Z372
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5C Monoclonal Antibody, Unconjugated (TMAB-12006) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5C Monoclonal Antibody, Unconjugated (TMAB-12006) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5C Monoclonal Antibody, Unconjugated (TMAB-12006) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5C Monoclonal Antibody, Unconjugated (TMAB-12006) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. 25 μg total protein per lane of various lysates (see on figure) probed with RAB5C monoclonal antibody, unconjugated (TMAB-12006) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane. Early endosome membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundProtein transport. Probably involved in vesicular traffic.
Antigen Details
Immunogen
A synthesized peptide: human Rab5C
Antigen Species
Human
Gene Name
RAB5C
Gene ID
Protein Name
Ras-related protein Rab-5C
Uniprot ID
Function
Protein transport. Probably involved in vesicular traffic.
Chemical Properties
Molecular WeightTheoretical: 23 kDa. Actual: 25 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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