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Anti-QPRTase Polyclonal Antibody
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Catalog No. TMAB-01599
Anti-QPRTase Polyclonal Antibody is a Rabbit antibody targeting QPRTase. Anti-QPRTase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-QPRTase Polyclonal Antibody
Anti-QPRTase Polyclonal Antibody
😃Good
Catalog No. TMAB-01599
Anti-QPRTase Polyclonal Antibody is a Rabbit antibody targeting QPRTase. Anti-QPRTase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-QPRTase Polyclonal Antibody is a Rabbit antibody targeting QPRTase. Anti-QPRTase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Quinolinate phosphoribosyltransferase; QPRT/QAPRTase) Polyclonal Antibody, Unconjugated (TMAB-01599) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Liver (mouse) Lysate at 40 μg Primary: Anti-QPRT/QAPRTase (TMAB-01599) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa 3. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Large intestine tissue lysates Lane 3: Mouse Kidney tissue lysates Primary: Anti-QPRT/QAPRTase (TMAB-01599) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 28 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Hexamer formed by 3 homodimers. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Research Background | Quinolinate phosphoribosyltransferase (QPRTase) is a major enzyme in the catabolism of quinolinate. Quinolinate is an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide, and pyrophosphate. Catabolism of quinolinate is vital due to the quinolinate’s neurotoxicity. Increased levels of quinolinate have been linked to neurodegenerative symptoms associated with meningitis and AIDS. QAPRTase has a seven-stranded alpha/beta-barrel domain which is similar in structure to the eight-stranded alpha/beta-barrel enzymes. The protein possesses a novel fold in comparison to other members of the PRTase family. This fold comprises a structure combining two domains. The structure is part alpha/beta barrel-like domain, and part alpha/beta N-terminal domain. |
| Immunogen | KLH conjugated synthetic peptide: human QPRT/QAPRTase |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Amino acid metabolism,Neurodegenerative disease,Alzheimer's disease,Huntington's disease,Neurodegenerative disease,Amino Acids |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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