Anti-QARS/Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody is a Rabbit antibody targeting QARS/Glutamyl Prolyl tRNA synthetase/ProRS. Anti-QARS/Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated (TMAB-11964) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-QARS (TMAB-11964) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 171 kD
Observed band size: 171 kD
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (TMAB-11964) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (TMAB-11964) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (TMAB-11964) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ProRS

Human

QARS1

Glutamine--tRNA ligase

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.