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Anti-PYGM Polyclonal Antibody
😃Good
Catalog No. TMAB-01598
Alias PYGM, phosphorylase glycogen muscle(McArdle syndrome glycogen storage disease type V), Myophosphorylase, Glycogen phosphorylase muscle form Muscpho
Anti-PYGM Polyclonal Antibody is a Rabbit antibody targeting PYGM. Anti-PYGM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PYGM Polyclonal Antibody
Anti-PYGM Polyclonal Antibody
😃Good
Catalog No. TMAB-01598Alias PYGM, phosphorylase glycogen muscle(McArdle syndrome glycogen storage disease type V), Myophosphorylase, Glycogen phosphorylase muscle form Muscpho
Anti-PYGM Polyclonal Antibody is a Rabbit antibody targeting PYGM. Anti-PYGM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PYGM Polyclonal Antibody is a Rabbit antibody targeting PYGM. Anti-PYGM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | PYGM, phosphorylase glycogen muscle(McArdle syndrome glycogen storage disease type V), Myophosphorylase, Glycogen phosphorylase muscle form Muscpho |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (TMAB-01598) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (TMAB-01598) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Rat Muscle tissue lysates Primary: Anti-PYGM (TMAB-01598) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 97 kDa 4. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (TMAB-01598) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (TMAB-01598) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (TMAB-01598) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. |
| Immunogen | KLH conjugated synthetic peptide: human PYGM |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Metabolism of carbohydrates,Response to hypoxia,Carbohydrate metabolism,Energy Metabolism,Hypoxia,Energy Metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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