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Anti-Pumilio 1 Antibody (3G125)
😃Good
Catalog No. TMAC-03472
Alias PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM
Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in WB,ICC,IF,IHC,FCM,IP.
Anti-Pumilio 1 Antibody (3G125)
Anti-Pumilio 1 Antibody (3G125)
😃Good
Catalog No. TMAC-03472Alias PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM
Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in WB,ICC,IF,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in WB,ICC,IF,IHC,FCM,IP. |
| Synonyms | PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin. 6. ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human Pumilio 1 aa 1-200 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 126 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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