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Anti-Pumilio 1 Antibody (3G125) (Synonyms: PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio ...)

Catalog No. TMAC-03472 Copy Product Info
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Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in FCM,ICC,IF,IHC,IP,WB.

Anti-Pumilio 1 Antibody (3G125)

Copy Product Info
🥰Excellent
Catalog No. TMAC-03472
Synonyms PUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM

Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in FCM,ICC,IF,IHC,IP,WB.

Anti-Pumilio 1 Antibody
(3G125)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Pumilio 1 Antibody (3G125) is a Rabbit antibody targeting Pumilio 1. Anti-Pumilio 1 Antibody (3G125) can be used in FCM,ICC,IF,IHC,IP,WB.
SynonymsPUML1, PUML 1, Pumilio1, pumilio homolog 1 (Drosophila), Pumilio homolog 1, PUMH1, PUMH 1, PUMH, PUM1_HUMAN, Pum1, PUM L1, PUM 1, KIAA0099, HsPUM
Ig Type
IgG
Clone
3G125
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
6. ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundSequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human Pumilio 1 aa 1-200
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 126 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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