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Anti-PUMA Antibody (5V529)
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Catalog No. TMAC-03471
Alias PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3
Anti-PUMA Antibody (5V529) is a Rabbit antibody targeting PUMA. Anti-PUMA Antibody (5V529) can be used in FCM,ICC/IF,IHC,WB.
Anti-PUMA Antibody (5V529)
Anti-PUMA Antibody (5V529)
😃Good
Catalog No. TMAC-03471Alias PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3
Anti-PUMA Antibody (5V529) is a Rabbit antibody targeting PUMA. Anti-PUMA Antibody (5V529) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-PUMA Antibody (5V529) is a Rabbit antibody targeting PUMA. Anti-PUMA Antibody (5V529) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | PUMA/JFY1, PUMA alpha, PUMA, p53 Upregulated Modulator of Apoptosis, p53 up regulated modulator of apoptosis, JFY1, JFY 1, bcl-2 binding component 3, BCL2 binding component 3, BCL 2 binding component 3, BBC3_RAT, BBC3, BBC 3 |
| Ig Type | IgG |
| Clone | 5V529 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PUMA on different lysates using anti-PUMA antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: K562. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin. 6. ICC staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 18 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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